h2-eb1 molecule alleviates allergic rhinitis symptoms of h2-eb1 knockout mice

background:   h2-eb1 molecule which is the homolog of human hla-drb1 is proposed to be associated with allergic rhinitis (ar). construction of   h2-eb1 knockout animal models provides a tool to elucidate the role of h2-eb1 and ar pathogenesis. objective:   to establish the h2-eb1 knockout model and investigate the h2-eb1 functions in   h2-eb1 knockout mice as a model of ar. methods: the cre/loxp system and es gene knockout technology were applied to create heterozygous   h2-eb1 (+/-) knockout mice and their offspring of knockout homozygous(-/-), heterozygous (+/-) and wild type (+/+)   h2-eb1 mice. after identification, offspring of heterozygous (+/-) and homozygous (-/-)   h2-eb1 knockout mice were randomly selected to establish ar models to demonstrate the role of   h2-eb1 in ar pathogenesis. results: the h2-eb1 knockout mice model was successfully established. the reproduction and feeding of the homozygous (   -/-) h2-eb1 knockout mice were successful. compared with the control group, the serum ova-ige and il-4 levels significantly increased, while ifn-γ levels significantly dropped (p